Development of antimicrobials against Acanthamoeba
نویسندگان
چکیده
منابع مشابه
Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba.
We have developed and optimized a 96-well microtiter plate assay, based on the reduction of alamarBlue, to assess the efficacies of much needed new antimicrobials against Acanthamoeba species. This assay has been optimized for determination of drug efficacy against two potentially pathogenic species, Acanthamoeba castellanii and Acanthamoeba polyphaga, and has been validated by comparison of th...
متن کاملThe Development of Drugs against Acanthamoeba Infections.
For the past several decades, there has been little improvement in the morbidity and mortality associated with Acanthamoeba keratitis and Acanthamoeba encephalitis, respectively. The discovery of a plethora of antiacanthamoebic compounds has not yielded effective marketed chemotherapeutics. The rate of development of novel antiacanthamoebic chemotherapies of translational value and the lack of ...
متن کاملContact lens solution efficacy against Acanthamoeba castellani.
PURPOSE Acanthamoeba castellani, ATCC 30234, cysts, and trophozoites after a 6-hour exposure. METHODS Trophozoite cultures were prepared at Bio-Concept Laboratories in vented tissue culture flasks containing peptone yeast glucose broth by incubation (35 degrees C +/- 1 degrees C for 11 days). Cyst suspensions were prepared by incubation of trophozoites in phosphate-buffered saline plus heat-k...
متن کاملMycobacterium avium grown in Acanthamoeba castellanii is protected from the effects of antimicrobials.
Mycobacterium avium is a common cause of systemic bacterial infection in patients with AIDS. Infection with M. avium has been linked to bacterial colonization of domestic water supplies and commonly occurs through the gastrointestinal tract. Acanthamoeba castellanii, a waterborne protozoan, may serve as an environmental host for M. avium. It has been shown that growth of M. avium in amoebae enh...
متن کاملIn vitro activity of Trigonella foenum graecum seeds against a clinical strain of Acanthamoeba genotype T4
Acanthamoeba keratitis (AK) is a sight-threatening infection of the cornea disease that often presents with a lengthy and not fully effective treatment. Current therapeutic options against Acanthamoeba are not very effective against the cyst. Calibrated trophozoite/cyst suspension was incubated with the same volume of serial dilutions of the Trigonella foenum graecum aqueous extract (200, 250, ...
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ژورنال
عنوان ژورنال: Access Microbiology
سال: 2019
ISSN: 2516-8290
DOI: 10.1099/acmi.ac2019.po0469